pJB010
(Plasmid
#22616)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTZ19R
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 2863
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Vector typephagemid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman genomic PLP promoter region
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SpeciesH. sapiens (human)
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Insert Size (bp)4200
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GenBank IDNG_008863
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Entrez GenePLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13 Forward 20 primer
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains Hu PLP 5' upstream region, promoter region and Exon1 from Lambda (to +85)
This PLP DNA cloned by Hudson lab
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJB010 was a gift from Lynn Hudson (Addgene plasmid # 22616 ; http://n2t.net/addgene:22616 ; RRID:Addgene_22616) -
For your References section:
Identification of cis-regulatory elements in the myelin proteolipid protein (PLP) gene. Berndt JA, Kim JG, Hudson LD. J Biol Chem. 1992 Jul 25. 267(21):14730-7. PubMed 1378839