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pJB010
(Plasmid #22616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTZ19R
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 2863
  • Vector type
    phagemid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human genomic PLP promoter region
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • GenBank ID
    NG_008863
  • Entrez Gene
    PLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 Forward 20 primer
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains Hu PLP 5' upstream region, promoter region and Exon1 from Lambda (to +85)

This PLP DNA cloned by Hudson lab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB010 was a gift from Lynn Hudson (Addgene plasmid # 22616 ; http://n2t.net/addgene:22616 ; RRID:Addgene_22616)

  • For your References section:

    Identification of cis-regulatory elements in the myelin proteolipid protein (PLP) gene. Berndt JA, Kim JG, Hudson LD. J Biol Chem. 1992 Jul 25. 267(21):14730-7. PubMed 1378839
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