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Addgene

pPLP1.5
(Plasmid #22614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC8
  • Backbone size w/o insert (bp) 2665
  • Vector type
    E. Coli phagemid vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human Proteolipid Protein cDNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • GenBank ID
    AK295388
  • Entrez Gene
    PLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 1500bp Human Proteolipid protein (PLP) was subcloned from a lambda gt11 human brainstem cDNA library

This PLP DNA cloned by Hudson lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPLP1.5 was a gift from Lynn Hudson (Addgene plasmid # 22614 ; http://n2t.net/addgene:22614 ; RRID:Addgene_22614)

  • For your References section:

    Myelin-specific proteolipid protein is expressed in myelinating Schwann cells but is not incorporated into myelin sheaths. Puckett C, Hudson L, Ono K, Friedrich V, Benecke J, Dubois-Dalcq M, Lazzarini RA. J Neurosci Res. 1987 . 18(4):511-8. 10.1002/jnr.490180402 PubMed 2449540
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