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Addgene

pAM289
(Plasmid #226126)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226126 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET DUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Npl4 D460K/Y461A
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    D460K/Y461A
  • Entrez Gene
    NPL4 (a.k.a. YBR170C, HRD4)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM289 was a gift from Andreas Martin (Addgene plasmid # 226126 ; http://n2t.net/addgene:226126 ; RRID:Addgene_226126)

  • For your References section:

    The Ufd1 cofactor determines the linkage specificity of polyubiquitin chain engagement by the AAA+ ATPase Cdc48. Williams C, Dong KC, Arkinson C, Martin A. Mol Cell. 2023 Mar 2;83(5):759-769.e7. doi: 10.1016/j.molcel.2023.01.016. Epub 2023 Feb 2. 10.1016/j.molcel.2023.01.016 PubMed 36736315
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