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Addgene

pAM285
(Plasmid #226123)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET DUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-3C-Cdc48 W561A/Y562A
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    W561A/Y562A
  • Entrez Gene
    CDC48 (a.k.a. YDL126C)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM285 was a gift from Andreas Martin (Addgene plasmid # 226123 ; http://n2t.net/addgene:226123 ; RRID:Addgene_226123)

  • For your References section:

    The Ufd1 cofactor determines the linkage specificity of polyubiquitin chain engagement by the AAA+ ATPase Cdc48. Williams C, Dong KC, Arkinson C, Martin A. Mol Cell. 2023 Mar 2;83(5):759-769.e7. doi: 10.1016/j.molcel.2023.01.016. Epub 2023 Feb 2. 10.1016/j.molcel.2023.01.016 PubMed 36736315
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