pcs2-cDELE1(142-515)-sumo
(Plasmid
#226107)
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PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDELE1
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SpeciesH. sapiens (human)
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Mutationencodes only residues 142-515 of DELE1 (representing the mitochondrially imported and cleaved form of DELE1)
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Entrez GeneDELE1 (a.k.a. DELE, DELE1(L), KIAA0141)
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Tag
/ Fusion Protein
- Sumo (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcs2-cDELE1(142-515)-sumo was a gift from Michael Rape (Addgene plasmid # 226107 ; http://n2t.net/addgene:226107 ; RRID:Addgene_226107) -
For your References section:
Stress response silencing by an E3 ligase mutated in neurodegeneration. Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rape M. Nature. 2024 Feb;626(8000):874-880. doi: 10.1038/s41586-023-06985-7. Epub 2024 Jan 31. 10.1038/s41586-023-06985-7 PubMed 38297121