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pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry
(Plasmid #226101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcs2-GFP-IRES-mCherry
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HRI
  • Species
    H. sapiens (human)
  • Mutation
    SIFI degron helices 1 & 2 deleted (Δ61-112)
  • Entrez Gene
    EIF2AK1 (a.k.a. HCR, HRI, LEMSPAD, hHRI)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned into BsmBI digested pcs2-GFP-IRES-mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry was a gift from Michael Rape (Addgene plasmid # 226101 ; http://n2t.net/addgene:226101 ; RRID:Addgene_226101)

  • For your References section:

    Stress response silencing by an E3 ligase mutated in neurodegeneration. Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rape M. Nature. 2024 Feb;626(8000):874-880. doi: 10.1038/s41586-023-06985-7. Epub 2024 Jan 31. 10.1038/s41586-023-06985-7 PubMed 38297121
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