pcs2-GFP-IRES-mCherry
(Plasmid
#226098)
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Purpose(Empty Backbone) Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Conceptually based on work done by Dr. Stephen J. Elledge (HMS)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcs2-GFP-IRES-mCherry was a gift from Michael Rape (Addgene plasmid # 226098 ; http://n2t.net/addgene:226098 ; RRID:Addgene_226098) -
For your References section:
Stress response silencing by an E3 ligase mutated in neurodegeneration. Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rape M. Nature. 2024 Feb;626(8000):874-880. doi: 10.1038/s41586-023-06985-7. Epub 2024 Jan 31. 10.1038/s41586-023-06985-7 PubMed 38297121
Map uploaded by the depositor.
