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Addgene

MCP/PCP red RNA Lantern
(Plasmid #226097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone size w/o insert (bp) 5405
  • Total vector size (bp) 8083
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG
  • Insert Size (bp)
    2714
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGGAGACCCAAGCTTatgggcccaaaaaagaaaagaaaagtt
  • 3′ sequencing primer CGGCCGCCAGTGTGATGGATATCTGCAGAATTCTTACTTGTCATCGTCGTCCTTGTAGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.07.02.498144 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCP/PCP red RNA Lantern was a gift from Jennifer Prescher (Addgene plasmid # 226097 ; http://n2t.net/addgene:226097 ; RRID:Addgene_226097)

  • For your References section:

    A modular platform for bioluminescent RNA tracking. Halbers LP, Cole KH, Ng KK, Fuller EB, Chan CET, Callicoatte C, Metcalfe M, Chen CC, Barhoosh AA, Reid-McLaughlin E, Kent AD, Torrey ZR, Steward O, Luptak A, Prescher JA. Nat Commun. 2024 Nov 18;15(1):9992. doi: 10.1038/s41467-024-54263-5. 10.1038/s41467-024-54263-5 PubMed 39557883
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