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Addgene

PB_EF1a-CasRx-msfGFP-2A-Blast
(Plasmid #226010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasRx RNA-targeting nuclease
  • Tag / Fusion Protein
    • msfGFP (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This does not include a guideRNA (see separate lenti plasmid: pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI, Addgene #226011) and needs to be co-transfected with a plasmid encoding the Piggybac transposase for insertion into the genome.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB_EF1a-CasRx-msfGFP-2A-Blast was a gift from Silvana Konermann (Addgene plasmid # 226010 ; http://n2t.net/addgene:226010 ; RRID:Addgene_226010)

  • For your References section:

    Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting. Wei J, Lotfy P, Faizi K, Baungaard S, Gibson E, Wang E, Slabodkin H, Kinnaman E, Chandrasekaran S, Kitano H, Durrant MG, Duffy CV, Pawluk A, Hsu PD, Konermann S. Cell Syst. 2023 Dec 20;14(12):1087-1102.e13. doi: 10.1016/j.cels.2023.11.006. Epub 2023 Dec 12. 10.1016/j.cels.2023.11.006 PubMed 38091991
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