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Addgene

pEF2-EFGR-hAxl
(Plasmid #225997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF2
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human EGFR N-Terminal human Axl C-Terminal Fusion
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3272
  • GenBank ID
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Entrez Gene
    AXL (a.k.a. ARK, AXL3, JTK11, Tyro7, UFO)
  • Promoter EF1-a

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ttcttccatttcaggtgtcg
  • 3′ sequencing primer gtcgaggctgatcagcgagc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF2-EFGR-hAxl was a gift from Raymond Birge & Sergei Kotenko (Addgene plasmid # 225997 ; http://n2t.net/addgene:225997 ; RRID:Addgene_225997)

  • For your References section:

    Normalization of TAM post-receptor signaling reveals a cell invasive signature for Axl tyrosine kinase. Kimani SG, Kumar S, Davra V, Chang YJ, Kasikara C, Geng K, Tsou WI, Wang S, Hoque M, Bohac A, Lewis-Antes A, De Lorenzo MS, Kotenko SV, Birge RB. Cell Commun Signal. 2016 Sep 6;14(1):19. doi: 10.1186/s12964-016-0142-1. 10.1186/s12964-016-0142-1 PubMed 27595981
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