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Addgene

pAG1guide_RUBY_EggCas9
(Plasmid #225983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    35s:RUBY and pRU53
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    RUBY (35s:RUBY)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Guide RNA against AtAGAMOUS1
  • gRNA/shRNA sequence
    gttttagagctagaaatagcaagttaaaataaggc
  • Species
    A. thaliana (mustard weed)
  • Promoter 35s promoter to Drive RUBY and DD45p to drive Cas9

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG1guide_RUBY_EggCas9 was a gift from Imran Siddiqi (Addgene plasmid # 225983 ; http://n2t.net/addgene:225983 ; RRID:Addgene_225983)
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