pRC072
(Plasmid
#225982)
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PurposeJ23110_buj_sfGFP_buj_mRFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepsc101**
- Total vector size (bp) 5187
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP (with buj RBS), mRFP (with buj RBS)
- Promoter J23110
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC072 was a gift from Jesse Zalatan (Addgene plasmid # 225982 ; http://n2t.net/addgene:225982 ; RRID:Addgene_225982) -
For your References section:
CRISPR-Cas tools for simultaneous transcription & translation control in bacteria. Cardiff RAL, Faulkner ID, Beall JG, Carothers JM, Zalatan JG. Nucleic Acids Res. 2024 May 22;52(9):5406-5419. doi: 10.1093/nar/gkae275. 10.1093/nar/gkae275 PubMed 38613390