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Addgene

pRC065d
(Plasmid #225979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a
  • Total vector size (bp) 4982
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dRxCas13d
  • Promoter J23107

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    buj1 crRNA (non-cognate DR) targets the unstructured RBS
  • Alt name
    gRNA: AGAACGTCTTCGCTACTCGCCATGGTA
  • Species
    Synthetic
  • Promoter J23119

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC065d was a gift from Jesse Zalatan (Addgene plasmid # 225979 ; http://n2t.net/addgene:225979 ; RRID:Addgene_225979)

  • For your References section:

    CRISPR-Cas tools for simultaneous transcription & translation control in bacteria. Cardiff RAL, Faulkner ID, Beall JG, Carothers JM, Zalatan JG. Nucleic Acids Res. 2024 May 22;52(9):5406-5419. doi: 10.1093/nar/gkae275. 10.1093/nar/gkae275 PubMed 38613390
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