pRC055
(Plasmid
#225977)
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PurposedRxCas13d expressed from pTet promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15a
- Total vector size (bp) 5494
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedRxCas13d
- Promoter pTet
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC055 was a gift from Jesse Zalatan (Addgene plasmid # 225977 ; http://n2t.net/addgene:225977 ; RRID:Addgene_225977) -
For your References section:
CRISPR-Cas tools for simultaneous transcription & translation control in bacteria. Cardiff RAL, Faulkner ID, Beall JG, Carothers JM, Zalatan JG. Nucleic Acids Res. 2024 May 22;52(9):5406-5419. doi: 10.1093/nar/gkae275. 10.1093/nar/gkae275 PubMed 38613390