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Addgene

pQE-9-RTXWTM-X2S-C
(Plasmid #225967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-9
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RTXWTM-X2S-C
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-9-RTXWTM-X2S-C was a gift from Danielle Mai (Addgene plasmid # 225967 ; http://n2t.net/addgene:225967 ; RRID:Addgene_225967)

  • For your References section:

    Sequence-defined structural transitions by calcium-responsive proteins. Chang M, Huang W, Shambharkar G, Hernandez K, Mai DJ. Polym Chem. 2024. doi: https://doi.org/10.1039/D4PY00907J 10.1039/D4PY00907J
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