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pCF142_U6-sgRNA
(Plasmid #225960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCF142
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6-sgRNA (recipient)
  • gRNA/shRNA sequence
    ggagacggaggacgacgaacgtctct
  • Species
    Spacer (Esp3I)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.06.25.600517 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF142_U6-sgRNA was a gift from Christof Fellmann (Addgene plasmid # 225960 ; http://n2t.net/addgene:225960 ; RRID:Addgene_225960)

  • For your References section:

    Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes. Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR. bioRxiv [Preprint]. 2024 Jun 26:2024.06.25.600517. doi: 10.1101/2024.06.25.600517. 10.1101/2024.06.25.600517 PubMed 38979269
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