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Addgene

EKAREN5-gl
(Plasmid #225958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSII lentiviral vector
  • Total vector size (bp) 12385
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EKAREN5-gl
  • Species
    Aequorea victoria
  • Insert Size (bp)
    3258
  • Promoter EF-1-alpha promoter
  • Tag / Fusion Protein
    • nls localization motif (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    EKAREN5(Hugo Snippert lab)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EKAREN5-gl was a gift from Toru Hiratsuka (Addgene plasmid # 225958 ; http://n2t.net/addgene:225958 ; RRID:Addgene_225958)

  • For your References section:

    A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription. Weisheng Z, Nakayama J, Inomata Y, Higashiyama S, Hiratsuka T. Cell Struct Funct. 2024 Dec 18. doi: 10.1247/csf.24070. 10.1247/csf.24070 PubMed 39694501
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