Flag-HA-USP16
(Plasmid #22595)

• Depositing Lab: Wade Harper
• Publication: Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Backbone

• Vector backbone: pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro
• Backbone manufacturer: Harper_Lab
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: USP16
• Alt name: ubiquitin specific peptidase 16
• Alt name: UBP-M
• Species: H. sapiens (human)
• Mutation: None
• GenBank ID: BC030777.2
• Entrez Gene: USP16 (a.k.a. MSTP039, UBP-M, UBPM)
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: GATEWAY_LR (unknown if destroyed)
• 3′ cloning site: GATEWAY_LR (unknown if destroyed)
• 5′ sequencing primer: CMV_forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Marc_Vidal_Orfeome_collection
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry