pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald
(Plasmid
#225947)
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PurposeLentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-SFFV-MCS-WPRE-UbC-Emerald (Addgene #211796)
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Backbone manufacturerBart Nieuwenhuis
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCKAP4
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Alt nameCytoskeleton Associated Protein 4
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SpeciesH. sapiens (human)
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Insert Size (bp)1809
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MutationS3E S17E S19E
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Entrez GeneCKAP4 (a.k.a. CLIMP-63, CLIMP63, ERGIC-63, p63)
- Promoter SFFV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameEmerald
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Alt nameEmGFP
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SpeciesA. victoria
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Insert Size (bp)717
- Promoter UbC
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald was a gift from Richard Eva & Bart Nieuwenhuis & Evan Reid (Addgene plasmid # 225947 ; http://n2t.net/addgene:225947 ; RRID:Addgene_225947)