pLV-SFFV-KTN1-WPRE-UbC-Emerald
(Plasmid
#225941)
-
PurposeLentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 225941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLV-SFFV-MCS-WPRE-UbC-Emerald (Addgene #211796)
-
Backbone manufacturerBart Nieuwenhuis
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameKTN1
-
Alt nameKinectin 1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)4074
-
Entrez GeneKTN1 (a.k.a. CG1, KNT, MU-RMS-40.19)
- Promoter SFFV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameEmerald
-
Alt nameEmGFP
-
SpeciesA. victoria
- Promoter UbC
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV-SFFV-KTN1-WPRE-UbC-Emerald was a gift from Richard Eva & Bart Nieuwenhuis & Evan Reid (Addgene plasmid # 225941 ; http://n2t.net/addgene:225941 ; RRID:Addgene_225941)