10_T7-SP-CD8tm-mScarlet3
(Plasmid
#225937)
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PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTwist
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Backbone manufacturerTwist Bioscience
- Total vector size (bp) 4060
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Vector typeIn vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSP-CD8tm-mScarlet3
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SpeciesSynthetic
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Insert Size (bp)1839
- Promoter T7
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
10_T7-SP-CD8tm-mScarlet3 was a gift from Michalis Averof (Addgene plasmid # 225937 ; http://n2t.net/addgene:225937 ; RRID:Addgene_225937) -
For your References section:
A toolkit for testing membrane localisation tags across species. Karapidaki I, Momose T, Zilliox M, Averof M. bioRxiv 2024.11.12.623055 10.1101/2024.11.12.623055
