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pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR
(Plasmid #225925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS3
  • Backbone manufacturer
    Anuj Sharma
  • Backbone size w/o insert (bp) 3481
  • Total vector size (bp) 10899
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LGC-47
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1230
  • Entrez Gene
    lgc-47 (a.k.a. CELE_F47A4.1)
  • Promoter tdc-1

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR was a gift from Andrew Leifer (Addgene plasmid # 225925 ; http://n2t.net/addgene:225925 ; RRID:Addgene_225925)

  • For your References section:

    An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. Kumar S, Sharma AK, Leifer AM. bioRxiv [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 10.1101/2024.03.21.586204. 10.1101/2024.03.21.586204 PubMed 38585821
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