pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR
(Plasmid
#225924)
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PurposeExpressing LGC-47 in AVA neuron
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAS3
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Backbone manufacturerAnuj Sharma
- Backbone size w/o insert (bp) 3481
- Total vector size (bp) 8814
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLGC-47
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SpeciesC. elegans (nematode)
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Insert Size (bp)1230
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Entrez Genelgc-47 (a.k.a. CELE_F47A4.1)
- Promoter rig-3
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer caggaaacagctatgac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR was a gift from Andrew Leifer (Addgene plasmid # 225924 ; http://n2t.net/addgene:225924 ; RRID:Addgene_225924) -
For your References section:
An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. Kumar S, Sharma AK, Leifer AM. bioRxiv [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 10.1101/2024.03.21.586204. 10.1101/2024.03.21.586204 PubMed 38585821