pUCmini-UGCG-EGFP
(Plasmid
#225878)
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PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUCmini
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCmini-UGCG-EGFP was a gift from Yohei Sato (Addgene plasmid # 225878 ; http://n2t.net/addgene:225878 ; RRID:Addgene_225878) -
For your References section:
UDP-glucose ceramide glucosyltransferase specifically upregulated in plasmacytoid dendritic cells regulates type I interferon production upon CpG stimulation. Sato Y, Osada E, Ushiki T, Maeda T, Manome Y. Biochem Biophys Res Commun. 2024 Sep 18;733:150703. doi: 10.1016/j.bbrc.2024.150703. 10.1016/j.bbrc.2024.150703 PubMed 39307111