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Addgene

Control-RB1-LRG-GFP
(Plasmid #225875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LRG-EGFP
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Control and RB1
  • gRNA/shRNA sequence
    GTAATCCTAGCACTTTTAGG and GCTCTGGGTCCTCCTCAGGA
  • Species
    H. sapiens (human)
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.06.13.495815 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Control-RB1-LRG-GFP was a gift from Janai Carr-Ascher (Addgene plasmid # 225875 ; http://n2t.net/addgene:225875 ; RRID:Addgene_225875)

  • For your References section:

    Genetic Screen in a Pre-Clinical Model of Sarcoma Development Defines Drivers and Therapeutic Vulnerabilities. Freeland J, Munoz M, O'Donnell E, Langerman J, Darrow M, Bergonio J, Suarez-Navarro J, Thorpe S, Canter R, Randall RL, Plath K, Carraway KL, Witte ON, Graeber TG, Carr-Ascher JR. Clin Cancer Res. 2024 Aug 23. doi: 10.1158/1078-0432.CCR-24-1238. 10.1158/1078-0432.CCR-24-1238 PubMed 39177582
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