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Addgene

pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD
(Plasmid #225872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H3.3(K36M) amino acids 1-44
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    amino acids 1-44 from H3.3 K36M
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Entrez Gene
    H3-3B (a.k.a. BRYLIB2, H3-3A, H3.3B, H3F3B)
  • Tags / Fusion Proteins
    • T2A-mCherry (C terminal on insert)
    • 3xAID HA (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD was a gift from Lacra Bintu (Addgene plasmid # 225872 ; http://n2t.net/addgene:225872 ; RRID:Addgene_225872)

  • For your References section:

    The H3.3 K36M oncohistone disrupts the establishment of epigenetic memory through loss of DNA methylation. Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, Wan J, Fang D, Bintu L. bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 10.1101/2023.10.13.562147. 10.1101/2023.10.13.562147 PubMed 37873347
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