pIgK-2.1-FLAG
(Plasmid
#225829)
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PurposeMouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG-tagged monoclonal antibodies for use as tag-specific ELISA standards.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225829 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIgK
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse anti-CGG IgK chain, Flag-tagged
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SpeciesM. musculus (mouse)
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Insert Size (bp)768
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIgK-2.1-FLAG was a gift from Gabriel Victora (Addgene plasmid # 225829 ; http://n2t.net/addgene:225829 ; RRID:Addgene_225829) -
For your References section:
Molecular fate-mapping of serum antibody responses to repeat immunization. Schiepers A, van 't Wout MFL, Greaney AJ, Zang T, Muramatsu H, Lin PJC, Tam YK, Mesin L, Starr TN, Bieniasz PD, Pardi N, Bloom JD, Victora GD. Nature. 2023 Mar;615(7952):482-489. doi: 10.1038/s41586-023-05715-3. Epub 2023 Jan 16. 10.1038/s41586-023-05715-3 PubMed 36646114