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Addgene

CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)
(Plasmid #225753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CROPseq-multi-Puro (pRW1441)
  • Modifications to backbone
    a T7 promoter is encoded within the 3' LTR
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500) was a gift from Paul Blainey (Addgene plasmid # 225753 ; http://n2t.net/addgene:225753 ; RRID:Addgene_225753)

  • For your References section:

    CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens. Walton RT, Qin Y, Blainey PC. bioRxiv 2024.03.17.585235 10.1101/2024.03.17.585235
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