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Addgene

pGF-GAS4X
(Plasmid #225751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGF2-NFAT
  • Backbone manufacturer
    SBI
  • Backbone size w/o insert (bp) 9630
  • Total vector size (bp) 9865
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    interferon gamma activating sequence (GAS)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    235
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site BHI (unknown if destroyed)
  • 5′ sequencing primer aattcaaaattttatcgat actagtctcgagaagagg 4xGAS-BHI_
  • 3′ sequencing primer tccatggtggcggatcc aacgaatgtcgagaggct 1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.doi.org/10.17632/g75gzdwbks.1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGF-GAS4X was a gift from Eun Hee Han (Addgene plasmid # 225751 ; http://n2t.net/addgene:225751 ; RRID:Addgene_225751)

  • For your References section:

    STAT1 as a tool for non-invasive monitoring of NK cell activation in cancer. Min JY, Kim HM, Lee H, Cho MY, Park HS, Lee SY, Park MS, Ha SK, Kim D, Jeong HG, Kim TD, Hong KS, Han EH. Commun Biol. 2024 Sep 30;7(1):1222. doi: 10.1038/s42003-024-06917-9. 10.1038/s42003-024-06917-9 PubMed 39349746
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