pMV261-igr3WT
(Plasmid
#225738)
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PurposeTo express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMV261
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMyc-igr3WT
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Alt nameRv3542c-Rv3541c-Rv3540c
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SpeciesMycobacterium tuberculosis
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Tag
/ Fusion Protein
- c-Myc (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMV261-igr3WT was a gift from Nicole Sampson (Addgene plasmid # 225738 ; http://n2t.net/addgene:225738 ; RRID:Addgene_225738)