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Addgene

pET28b-M2
(Plasmid #225735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mce3R
  • Alt name
    219-399 amino acids
  • Species
    Mycobacterium tuberculosis
  • Mutation
    aa 219-399
  • Tags / Fusion Proteins
    • 6xHis (C terminal on insert)
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-M2 was a gift from Nicole Sampson (Addgene plasmid # 225735 ; http://n2t.net/addgene:225735 ; RRID:Addgene_225735)

  • For your References section:

    Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an Asymmetric Four-Helix Bundle and Binds a Nonpalindrome Sequencedagger. Panagoda NT, Balazsi G, Sampson NS. ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 10.1021/acschembio.4c00687. Epub 2024 Nov 15. 10.1021/acschembio.4c00687 PubMed 39545866
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