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2M-T-Mce3R_K262A
(Plasmid #225728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mce3R
  • Alt name
    Rv1963c
  • Species
    Mycobacterium tuberculosis
  • Mutation
    K262A
  • Tag / Fusion Protein
    • 6xHis-MBP (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Discrepancy in insert: K406T. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2M-T-Mce3R_K262A was a gift from Nicole Sampson (Addgene plasmid # 225728 ; http://n2t.net/addgene:225728 ; RRID:Addgene_225728)

  • For your References section:

    Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an Asymmetric Four-Helix Bundle and Binds a Nonpalindrome Sequencedagger. Panagoda NT, Balazsi G, Sampson NS. ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 10.1021/acschembio.4c00687. Epub 2024 Nov 15. 10.1021/acschembio.4c00687 PubMed 39545866
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