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pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD
(Plasmid #225698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H3.3 (K27M)
  • Species
    H. sapiens (human)
  • Mutation
    K27M
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Entrez Gene
    H3-3B (a.k.a. BRYLIB2, H3-3A, H3.3B, H3F3B)
  • Tags / Fusion Proteins
    • 3xAID HA (C terminal on insert)
    • T2A-mCherry (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD was a gift from Lacra Bintu (Addgene plasmid # 225698 ; http://n2t.net/addgene:225698 ; RRID:Addgene_225698)

  • For your References section:

    The H3.3 K36M oncohistone disrupts the establishment of epigenetic memory through loss of DNA methylation. Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, Wan J, Fang D, Bintu L. bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 10.1101/2023.10.13.562147. 10.1101/2023.10.13.562147 PubMed 37873347
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