pLenti-osTIR1-9xMyc-Zeo
(Plasmid
#225696)
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PurposeLentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameosTIR1
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SpeciesOryza sativa
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Tag
/ Fusion Protein
- 9X Myc (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-osTIR1-9xMyc-Zeo was a gift from Lacra Bintu (Addgene plasmid # 225696 ; http://n2t.net/addgene:225696 ; RRID:Addgene_225696) -
For your References section:
The H3.3 K36M oncohistone disrupts the establishment of epigenetic memory through loss of DNA methylation. Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, Wan J, Fang D, Bintu L. bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 10.1101/2023.10.13.562147. 10.1101/2023.10.13.562147 PubMed 37873347