pMS18
(Plasmid
#225681)
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PurposeConfers puromycin resistance to Salpingoeca rosetta
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepEFL-Pac-Actin_3'UTR
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Alt namePac
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SpeciesSynthetic
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Insert Size (bp)1908
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.13.603360 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMS18 was a gift from Thibaut Brunet (Addgene plasmid # 225681 ; http://n2t.net/addgene:225681 ; RRID:Addgene_225681) -
For your References section:
A fast and robust gene knockout method for Salpingoeca rosetta clarifies the genetics of choanoflagellate multicellular development. Combredet C, Brunet T. bioRxiv 2024.07.13.603360 10.1101/2024.07.13.603360