pLVX-PERK(LD)-EGFP-HOTag3
(Plasmid
#225678)
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Purpose(Empty Backbone) Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLVX
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Backbone manufactureraddgene
- Backbone size (bp) 9001
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-PERK(LD)-EGFP-HOTag3 was a gift from Likun Wang (Addgene plasmid # 225678 ; http://n2t.net/addgene:225678 ; RRID:Addgene_225678) -
For your References section:
Deciphering ER stress-unfolded protein response relationship by visualizing unfolded proteins in the ER. Xu F, Wang L. Cell Rep. 2024 Jun 25;43(6):114358. doi: 10.1016/j.celrep.2024.114358. Epub 2024 Jun 11. 10.1016/j.celrep.2024.114358 PubMed 38865243
Map uploaded by the depositor.
