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Addgene

18065-M35-412
(Plasmid #225670)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225670 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-412
  • Backbone size w/o insert (bp) 5295
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dsEGFP
  • Alt name
    destabilized EGFP
  • Promoter S3x6-JunBp

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TGF-beta reporter construct using 6 repeats of upstream Smad3 binding region in JunB linked to JunB minimal promoter. Expresses EGFP destabilized by fusion to a mutated version of residues 422-461 of mouse ornithine decarboxylase, giving an in vivo half-life of ~1 hour.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    18065-M35-412 was a gift from Dominic Esposito (Addgene plasmid # 225670 ; http://n2t.net/addgene:225670 ; RRID:Addgene_225670)
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