18065-M10-366
(Plasmid
#225669)
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PurposeMammalian protein expression of a TGF-beta pathway reporter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-366
- Backbone size w/o insert (bp) 2745
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameffLluc2
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Alt namefirefly luciferase
- Promoter S3x6-JunBp
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TGF-beta pathway reporter construct using 6 repeats of the mouse upstream Smad3 binding region in JunB linked to JunB minimal promoter
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
18065-M10-366 was a gift from Dominic Esposito (Addgene plasmid # 225669 ; http://n2t.net/addgene:225669 ; RRID:Addgene_225669)