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Addgene

18065-E01
(Plasmid #225668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonr-258
  • Backbone size w/o insert (bp) 2726
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S3x6-JunBp
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1469

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
  • 3′ sequencing primer DR 23-mer (5'-TTGGGTAGCAGCGAAGTCGAGGC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Entry clone (4-1r): TGF-beta pathway reporter construct using 6 repeats of the mouse homolog of a high affinity Smad3 binding region identified 9kb upstream of the human JUNB promoter (from Ramachandran et al. 2018 https://doi.org/10.7554/elife.31756) linked to a JunB minimal promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    18065-E01 was a gift from Dominic Esposito (Addgene plasmid # 225668 ; http://n2t.net/addgene:225668 ; RRID:Addgene_225668)
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