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18065-M04-412
(Plasmid #225667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-412
  • Backbone size w/o insert (bp) 5295
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    dsmCherry
  • Alt name
    destabilized form of mCherry
  • Species
    Synthetic
  • Promoter mCMVp
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    delCD19
  • Species
    H. sapiens (human)
  • Mutation
    C-terminally truncated (aa 1-333 only)
  • Entrez Gene
    CD19 (a.k.a. B4, CVID3)
  • Promoter WPRE-SV40p

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    18065-M04-412 was a gift from Dominic Esposito (Addgene plasmid # 225667 ; http://n2t.net/addgene:225667 ; RRID:Addgene_225667)

  • For your References section:

    Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination. Sharma VP, Tang B, Wang Y, Duran CL, Karagiannis GS, Xue EA, Entenberg D, Borriello L, Coste A, Eddy RJ, Kim G, Ye X, Jones JG, Grunblatt E, Agi N, Roy S, Bandyopadhyaya G, Adler E, Surve CR, Esposito D, Goswami S, Segall JE, Guo W, Condeelis JS, Wakefield LM, Oktay MH. Nat Commun. 2021 Dec 15;12(1):7300. doi: 10.1038/s41467-021-27308-2. 10.1038/s41467-021-27308-2 PubMed 34911937
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