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Addgene

8522-M31-663
(Plasmid #225661)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225661 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDest-663
  • Backbone size w/o insert (bp) 7337
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dsCherry
  • Alt name
    destabilized form of mCherry
  • Species
    Synthetic
  • Promoter SORE6-mCMVp

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use plasmid 10279-M30-663 (Addgene # 225663) as matched control

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8522-M31-663 was a gift from Dominic Esposito (Addgene plasmid # 225661 ; http://n2t.net/addgene:225661 ; RRID:Addgene_225661)
  • For your References section:

    A flexible reporter system for direct observation and isolation of cancer stem cells. Tang B, Raviv A, Esposito D, Flanders KC, Daniel C, Nghiem BT, Garfield S, Lim L, Mannan P, Robles AI, Smith WI Jr, Zimmerberg J, Ravin R, Wakefield LM. Stem Cell Reports. 2015 Jan 13;4(1):155-169. doi: 10.1016/j.stemcr.2014.11.002. Epub 2014 Dec 11. 10.1016/j.stemcr.2014.11.002 PubMed 25497455