8522-M31-663
(Plasmid
#225661)
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PurposeLentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-663
- Backbone size w/o insert (bp) 7337
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedsCherry
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Alt namedestabilized form of mCherry
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SpeciesSynthetic
- Promoter SORE6-mCMVp
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use plasmid 10279-M30-663 (Addgene # 225663) as matched control
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
8522-M31-663 was a gift from Dominic Esposito (Addgene plasmid # 225661 ; http://n2t.net/addgene:225661 ; RRID:Addgene_225661) -
For your References section:
A flexible reporter system for direct observation and isolation of cancer stem cells. Tang B, Raviv A, Esposito D, Flanders KC, Daniel C, Nghiem BT, Garfield S, Lim L, Mannan P, Robles AI, Smith WI Jr, Zimmerberg J, Ravin R, Wakefield LM. Stem Cell Reports. 2015 Jan 13;4(1):155-169. doi: 10.1016/j.stemcr.2014.11.002. Epub 2014 Dec 11. 10.1016/j.stemcr.2014.11.002 PubMed 25497455