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Addgene

8138-E02
(Plasmid #225659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonr-215
  • Backbone size w/o insert (bp) 2228
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SORE6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    296

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer 6955 23-mer (5'-TTATTTGATGCCTGGCAGTTCCC-3')
  • 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Entry clone (4-5): SORE6 consists of 6 repeats of a composite SOX2/OCT4 response element from the human NANOG promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8138-E02 was a gift from Dominic Esposito (Addgene plasmid # 225659 ; http://n2t.net/addgene:225659 ; RRID:Addgene_225659)

  • For your References section:

    A flexible reporter system for direct observation and isolation of cancer stem cells. Tang B, Raviv A, Esposito D, Flanders KC, Daniel C, Nghiem BT, Garfield S, Lim L, Mannan P, Robles AI, Smith WI Jr, Zimmerberg J, Ravin R, Wakefield LM. Stem Cell Reports. 2015 Jan 13;4(1):155-169. doi: 10.1016/j.stemcr.2014.11.002. Epub 2014 Dec 11. 10.1016/j.stemcr.2014.11.002 PubMed 25497455
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