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Addgene

Ydf NanoLuc
(Plasmid #225656)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225656 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tabor Lab
  • Backbone manufacturer
    Gift from Jewett lab
  • Vector type
    Bacterial Expression ; Cell free expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NanoLuciferase
  • Species
    Synthetic
  • Insert Size (bp)
    555
  • Promoter PydfJ115
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Michael Jewett and Jeffrey Tabor Labs

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ydf NanoLuc was a gift from Neha Kamat (Addgene plasmid # 225656 ; http://n2t.net/addgene:225656 ; RRID:Addgene_225656)

  • For your References section:

    Engineering transmembrane signal transduction in synthetic membranes using two-component systems. Peruzzi JA, Galvez NR, Kamat NP. Proc Natl Acad Sci U S A. 2023 May 9;120(19):e2218610120. doi: 10.1073/pnas.2218610120. Epub 2023 May 1. 10.1073/pnas.2218610120 PubMed 37126679
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