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Addgene

pJL NrsS NarX Chimera
(Plasmid #225653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJL
  • Backbone manufacturer
    Gift from Jewett lab
  • Vector type
    Bacterial Expression ; Cell free expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NrsS-NarX
  • Species
    Synthetic
  • Insert Size (bp)
    1968
  • Mutation
    Transmembrane and sensing domain replaced to detect nickel (II)
  • Promoter T7
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL NrsS NarX Chimera was a gift from Neha Kamat (Addgene plasmid # 225653 ; http://n2t.net/addgene:225653 ; RRID:Addgene_225653)

  • For your References section:

    Engineering transmembrane signal transduction in synthetic membranes using two-component systems. Peruzzi JA, Galvez NR, Kamat NP. Proc Natl Acad Sci U S A. 2023 May 9;120(19):e2218610120. doi: 10.1073/pnas.2218610120. Epub 2023 May 1. 10.1073/pnas.2218610120 PubMed 37126679
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