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PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
(Plasmid #225649)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225649 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBELO_L6-PT3-T4_CAMR_TAN+
  • Backbone manufacturer
    IDT Iowa
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow in M9 glycerol media
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
  • Species
    Escherichia coli
  • Insert Size (bp)
    448
  • Promoter tetA, araBAD and LacO3O1 promoters
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+ was a gift from Andre Sanches Ribeiro (Addgene plasmid # 225649 ; http://n2t.net/addgene:225649 ; RRID:Addgene_225649)

  • For your References section:

    Transcription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation. Chauhan V, Baptista ISC, Arsh AM, Jagadeesan R, Dash S, Ribeiro AS. Biochemistry. 2024 Jul 12. doi: 10.1021/acs.biochem.4c00012. 10.1021/acs.biochem.4c00012 PubMed 38997112
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