pEMS2387
(Plasmid
#225633)
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PurposeAAV plasmid with Ple389 (ADORA2A MiniPromoter) driving expression of EmGFP. Contains WPRE.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEMS2131
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Backbone manufacturerElizabeth Simpson (Addgene plasmid # 111895)
- Backbone size w/o insert (bp) 4999
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsWe also recommend using “SURE” cells from Agilent, that the colonies are freshly picked, and that you limit the time to grow the culture. We typically transform the plasmid in the afternoon and take out the plate from the 37 degree incubator in the morning, we then pick the colonies and grow in LB for ~ 20 hrs
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePle389
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Alt nameADORA2A MiniPromoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1344
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Entrez GeneADORA2A (a.k.a. A2aR, ADORA2, RDC8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer oEMS6098 (GCCATGCTCTAGGAAGATCG)
- 3′ sequencing primer oEMS6163 (GGTTCTTGATCCCTTCTGAC) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2387 was a gift from Elizabeth Simpson (Addgene plasmid # 225633 ; http://n2t.net/addgene:225633 ; RRID:Addgene_225633) -
For your References section:
OnTarget: in silico design of MiniPromoters for targeted delivery of expression. Fornes O, Av-Shalom TV, Korecki AJ, Farkas RA, Arenillas DJ, Mathelier A, Simpson EM, Wasserman WW. Nucleic Acids Res. 2023 Jul 5;51(W1):W379-W386. doi: 10.1093/nar/gkad375. 10.1093/nar/gkad375 PubMed 37166953