pEMS2372
(Plasmid
#225630)
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PurposeHigh Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 3518
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePle389
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Alt nameADORA2A MiniPromoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1344
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Entrez GeneADORA2A (a.k.a. A2aR, ADORA2, RDC8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer M13-Fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2372 was a gift from Elizabeth Simpson (Addgene plasmid # 225630 ; http://n2t.net/addgene:225630 ; RRID:Addgene_225630) -
For your References section:
OnTarget: in silico design of MiniPromoters for targeted delivery of expression. Fornes O, Av-Shalom TV, Korecki AJ, Farkas RA, Arenillas DJ, Mathelier A, Simpson EM, Wasserman WW. Nucleic Acids Res. 2023 Jul 5;51(W1):W379-W386. doi: 10.1093/nar/gkad375. 10.1093/nar/gkad375 PubMed 37166953