pEND-225_Cacnes_mKO2
(Plasmid
#225612)
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PurposeReplicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBRESP36A
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Vector typeBacterial Expression, Synthetic Biology
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Selectable markersErythromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemKO2
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SpeciesSynthetic
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MutationCodon optimized for Cutibacterium acnes
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEND-225_Cacnes_mKO2 was a gift from Marc Güell (Addgene plasmid # 225612 ; http://n2t.net/addgene:225612 ; RRID:Addgene_225612) -
For your References section:
Synthetically-programmed antioxidant delivery by a domesticated skin commensal. Guillermo Nevot, Javier Santos-Moreno, Nil Campamà-Sanz, Lorena Toloza, Cristóbal Parra-Cid Patrick A.M. Jansen, Içvara Barbier, Rodrigo Ledesma-Amaro, Ellen van H. den Bogaard and Marc Güell. Cell Systems, 2025 10.1016/j.cels.2025.101169