pGEL1026
(Plasmid
#225597)
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Purpose(Empty Backbone) The STU backbone vector for rice genome editing is based on Mb3Cas12a-R (D172R)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTrans210
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Vector typeCRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEL1026 was a gift from Yong Zhang (Addgene plasmid # 225597 ; http://n2t.net/addgene:225597 ; RRID:Addgene_225597) -
For your References section:
PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato. Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou J, Zhong Z, An X, Qi Y, Zhang Y. Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486. 10.1111/pbi.14486 PubMed 39387219